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Genomics Unit

 

Tel: (84) 24 35575492

Address: Room 241, T8 Block - VNU University of Science

334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam

A-Genome hybridization to create new strains of the medicinal mushroom Cordyceps militaris with high yields of fruiting body formation
B-Genetic engineering of the filamentous fungus Aspergillus oryzae using a novel Agrobacterium tumefaciens-mediated transformation system
C-Characterization of the virulence factor-encoding genes in the citrus postharvest pathogen Penicillium digitatum using advanced genetic engineering tools

Curently employed staffs

Head: Dr. Tran Van Tuan, Lecturer

EducationPhD 2011, University of Goettingen, Germany

Postdoc: University of Goettingen, Germany (2011-2013)

SpecificationMolecular Microbiology, Fungal Genetics

Tel:  (84)24 35545492 

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Assoc.Prof.Dr. Nguyen Thi Hong Van, Lecturer

EducationPhD 2005, University of Greifswald, Germany

Specification: Genetics, Bioorganic chemistry

Tel:  (84)24 38584748 

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Dr. Do Thi Phuc, Lecturer

EducationPhD 2007, University of Greifswald, Germany

Postdoc: Mark Planck Institute of Molecular Plant Physiology, Germany (2007-2012)

Specification: Genetics, Molecular Plant Physiology

Tel: (84)24 38584748 

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Dr. Tran Duc Long, Lecturer

EducationPhD 2013, National University of Singapore

Specification: Genetics, Developmental Biology 

Tel: (84)2438584748 

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MSc. Tran Thi Thuy Anh, Researcher

EducationMSc 2005, VNU University of Science, Hanoi

Specification: Genetics, Biochemistry

Tel: (84)24 38584748 

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Research Interests and activities

Development of highly efficient systems for genetic transformation, recombinant gene expression and genome editing for some industrial fungi (Aspergillus oryzae, Aspergillus niger, Penicillium chrysogenum, Cordyceps militaris, Monascus purpureus, …) to generate hyper-producing mutants for enzymes/ proteins and bioactive substances.

Application of biotechnological advances to investigate molecular mechanism of plant infection by fungal pathogens and to produce new microbial products for efficiently controlling these phytopathogens.

Exploitation of genome databases and molecular biology tools for hybridization to create novel strains of the medicinal mushroom Cordyceps militaris with high yields of fruiting body formation.

Molecular characterization of abiotic and biotic stress tolerance in some important crops.

Screening/identification of gene mutations related to some human diseases and specific biomarkers for respective diagnostic assays.

Selected research projects

Development and application of novel binary vectors for genetic engineering of filamentous fungi by using Agrobacterium tumefaciens-mediated gene transfer (2015-2018). Ministry of Science and Technology, Vietnam, NAFOSTED, code: 106-NN.04-2014.75. PI: Dr. Tran Van Tuan.

Construction of a vector system for gene expression to improve efficacy of enzyme biosynthesis in the filamentous fungus Aspergillus oryzae (2014-2016). Vietnam National University, Hanoi, code: KLEPT.14.01. PI: Dr. Tran Van Tuan.

Generation of medicinal Monascus that does not synthesize citrinin by CRISPR/Cas9 in order to produce monacolin K (2016-2018), Vietnam National University, Hanoi, code: KLEPT.16.02. PI: Dr. Tran Duc Long.

Molecular characterization of salt tolerance in rice by analyzing Na+ transporter genes (2014-2017). Ministry of Science and Technology, Vietnam, NAFOSTED, code: 106-NN.02-2013.47. PI: Dr. Do Thi Phuc.

Investigation of DNA methylation on gene expression of SOS1 in relation to salt stress in contrasting rice genotypes (2014-2016). The World Academy of Science-TWAS, code: 14-2016RG/BIO/AS-I. PI: Dr. Do Thi Phuc.

Selected Publications

Van-Tuan Tran, Susanna A Braus-Stromeyer, Harald Kusch, Michael Reusche, Alexander Kaever, Anika Kuhn, Oliver Valerius, Manuel Landesfeind, Kathrin Abhauer, Maike Tech, Katharina Hoff, Tonatiuh Pena-Centeno, Mario Stanke, Volker Lipka, Gerhard H Braus (2014). Verticillium transcription activator of adhesion Vta2 suppresses microsclerotia formation and is required for systemic infection of plant roots. New Phytologist 202: 565-581.

Clara E Hoppenau, Van-Tuan Tran, Harald Kusch, Kathrin P Abhauer, Manuel Landesfeind, Peter Meinicke, Blagovesta Popova, Susanna A Braus-Stromeyer, Gerhard H Braus (2014).  Verticillium dahliae VdTH14, involved in thiazole biosynthesis, stress response and DNA repair functions, is required for vascular disease induction in tomato. Environmental and Experimental Botany 108: 14-22.

Phuong Anh Pham, Vanessa Wahl, Takayuki Tohge, Laise Rosado de Souza, Youjun Zhang, Phuc Thi Do, Justyna J. Olas, Mark Stitt, Wagner L. Araujo, and Alisdair R. Fernie (2015). Analysis of knockout mutants reveals non-redundant functions of poly(ADP-ribose) polymerase isoforms in ArabidopsisPlant Molecular Biology 89(4): 319-338.

Chi-Jan Lin, Christoph Sasse, Jenifer Gerke, Oliver Valerius, Henriette Irmer, Holm Frauendorf, Thorsten Heinekamp, Maria Strabburger, Van Tuan Tran, Britta Herzog, Susana A Braus-Stromeyer, Gerhard H Braus (2015). Transcription factor SomA is required for adhesion, development and virulence of the human pathogen Aspergillus fumigatusPLoS Pathogens 11(11): e1005205.

Khuyen Thi Nguyen, Quynh Ngoc Ho, Thu Ha Pham, Tuan Nghia Phan, Van Tuan Tran (2016). The construction and use of versatile binary vectors carrying pyrG auxotrophic marker and fluorescent reporter genes for Agrobacterium-mediated transformation of Aspergillus oryzaeWorld Journal of Microbiology and Biotechnology 32(12): 204.

Pham Quynh-Hoa, Tran Xuan-An, Nguyen Thi-Nha-Trang, Tran Thi-Thuy-Anh, Hoang Hai-Yen, Nguyen Thi-Hong-Van, Trang Thi-Hanh, Do Phuc Thi (2016). Investigation of polymorphisms in the coding region of OsHKT1 gene in relation to salinity in rice. Rice Science 23(6): 334-338.

Van Tuan Tran, Thi Binh Xuan Loc Do, Thi Khuyen Nguyen, Xuan Tao Vu, Bich Ngoc Dao, Hoai Ha Nguyen (2017). A simple, efficient and universal method for the extraction of genomic DNA from bacteria, yeasts, molds and microalgae suitable for PCR-based applications. Vietnam Journal of Science, Technology and Engineering 59(4): 66-74.

Khuyen Thi Nguyen, Quynh Ngoc Ho, Loc Thi Binh Xuan Do, Linh Thi Dam Mai, Duc-Ngoc Pham, Huyen Thi Thanh Tran, Diep Hong Le, Huy Quang Nguyen, Van-Tuan Tran (2017). A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation. World Journal of Microbiology and Biotechnology33(6): 107.

Tao Xuan Vu, Tho Tien Ngo, Linh Thi Dam Mai, Tri-Thuc Bui, Diep Hong Le, Ha Thi Viet Bui, Huy Quang Nguyen, Binh Xuan Ngo, Van-Tuan Tran (2018). A highly efficient Agrobacterium tumefaciens-mediated transformation system for the postharvest pathogen Penicillium digitatum using DsRed and GFP to visualize citrus host colonization. Journal of Microbiological Methods 144:134-144.