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Recombinant Proteins Unit

 

Tel:(84) 24-35575494

Address: Room 339, T8 Block- VNU University of Science

334 Nguyen Trai, Hanoi, Viet Nam

SDS-PAGE of recombinant Pfu DNA polymerase (A), SDS-PAGE of recombinant HIV-1 protease (B), Western blotting showing the expression of VP28 antigen protein of white spot syndrome virus (WSSV) on the surface of Bacillus subtilis spores (C) and protection of shrimps against WSSV by the recombinant Bacillus spores (D)


Curent staffs

Head: Prof. Dr. Phan Tuan Nghia, Senior Lecturer

Education: PhD: 1988, University of Hanoi (now known as VNU University of Science)

Postdoc: ICGEB, New Delhi, India (1995-1997), University of Rochester, NY, USA (1998- 2001)

Specification: Biochemistry and Molecular Biology

Tel: (84)2435575494

Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Dr. Nguyen Thi Hong Loan, Lecturer

Education: PhD 2012, VNU University of Science, Hanoi

Specification: Biochemistry

Tel: (84)24.35575494

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Dr. Dinh Nho Thai, Lecturer

Education: PhD 2009, Osaka University, Japan;

Postdoc: Arizona University (2009-2012)

Specification:  Biotechnology/Molecular Genetics

Tel:  (84)2438340072 

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Dr. Vu Thi Thu, Lecturer

Education: PhD 2010, Inje University, Korea;

Postdoc: Inje University (2010-2014)

Specification:  Medical physiology

Tel:    (84)903237808

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Dr. Khuong Thi Thu Huong, Lecturer

Education: PhD 2013, Marseille University, France;

Specification:  Plant technology

Tel:    (84)969043158

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Research Interests and activities

Production of recombinant proteins and enzymes of applications in medicine and agriculture.

Development of recombinant Bacillus subtilis spores as probiotic vaccine to protect shrimps against white spot syndrome virus attacks.

Investigation and development of HIV-1 protease inhibitors as potential drugs for HIV/AIDS treatment.

Development of assays for detection of some genetic diseases in humans.

Screening and assessment of drug effects on treatment of ischemic heart disease by targeting mitochondria.

Selected research projects                                                         

Study on production of recombinant Bacillus subtilis spores as probiotic vaccine for prevention of shrimps from white spot virus (2012-2015) funded by Ministry of Science and Technology, code: KC.04.09/11-15. PI: Prof. Dr. Phan Tuan Nghia.

Design of specific substrates for HIV-1 protease (2014-2016) funded Vietnam National University, code: KLEPT.14.02. PI: Dr. Nguyen Thi Hong Loan.

Development of a real-time PCR kit for detection and quantification of some common human mitochondrial genome mutations (2016-2018), funded by Vietnam National University, code KLEPT.16.03. PI: Prof. Dr. Phan Tuan Nghia.

Study on producing solution containing catalase enzyme and the extracted natural substances for application on anti-aging hair treatment (2016-2018), funded by Vietnam National University, code QG.16.82. PI: Dr. Dinh Nho Thai.

Study and screening of mitochondrial-targeted compounds with anti-ischemic-effects using ex vivo and in vitro models of myocardial ischemia in rats (2017-2020), funded by Ministry of Science and Technology, NAFOSTED, code 106-YS.06-2016.23, PI: Dr. Vu Thi Thu.

Selected Publications

Truong TH, Nguyen TVA, Nguyen THL, Pham VA, Phan TN (2014). Sensitive quantitation of mitochondrial mutation using new Taqman probes. Central European Journal of Medicine9: 839-848.

Nguyen TVA, Pham KC, Pham TTH, Pham LH, Nguyen HA, Dang TL, Huynh AH, Cutting SM, Phan TN (2014). Bacillus subtilis spores expressing the VP28 antigen: A potential oral treatment to protect Litopenaeus vannamei against white spot syndrome. FEMS Microbiology Letters 358: 202-208.

Nguyen THL, Nguyen TT, Vu TQ, Le TH, Pham BY, Trinh PL, Bui PT, Phan TN (2015). An efficient procedure for the expression and purification of HIV-1 protease from inclusion bodies. Protein Expression and Purification 116: 59-65.

Pham KC, Tran TTH, Doan VC, Le HP, Nguyen TVA, Nguyen HA, Huynh AH, Cutting SM, Phan TN (2016). Protection of Penaeus monodon against white spot syndrome by continuous oral administration of low concentration of Bacillus subtilis spores expressing the VP28 antigen. Letters in Applied Microbiology64:184-191. 

Truong TH, Nguyen TVA, Nguyen VL, Pham VA, Phan TN (2016). Screening of common point-mutations and discovery of new T14727C change in mitochondrial genome of Vietnamese encephalomyopathy patients. Mitochondrial DNA 27(1): 441-448.

Vu TT, Kim HK, Le TL, To TT, Nguyen QH, Kim SH, Kim N, Han J (2016). Necro X-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/DCN/TGFβ1/Smad2.  Korean Journal of Physiology and Pharmacology 20: 305-314.

Lee SC, Zhang J, Strom J, Yang D, Dinh NT, Kappeler K and Chen QM (2016). G-Quadruplex in Nrf2 5'UTR Regulates De Novo Nrf2 Protein Translation under Oxidative Stress. Molecular and Cell Biology 37: e00122-16.

Nguyễn Thị Hồng Loan, Trần Thị Thu Huyền, Đặng Thị Liễu, Phan Thị Lam Hồng, Phan Tuấn Nghĩa (2017). Thiết kế cơ chất peptide huỳnh quang đặc hiệu của protease HIV-1. Tạp chí Sinh học 39(1): 115-121.

Nga TTT, Hoa NQ, Tuyen HV, Tuyen DT, Thai DN (2016). Construction of yeast Pichia pastoris expressing the recombinant p53 as a secreted protein into culture. VNU Journal of Science: Natural Sciences and Technology 32(1S): 336-343.

Weerasinghe CAL, Bui TBH, Vu TT, Nguyen THL, Phung BK, Nguyen VM, Pham VA, Cao VH, Phan TN (2018). Leigh syndrome T8993C mtDNA mutation: heteroplasmy and the first clinical presentation in a Vietnamese family. Molecular Medicine Reports 17: 6919-6925.

Nguyen VD, Nguyen THL, Do LC, Vu VT, Thuong PT, Phan TN, 2018.A new saponin with anti-HIV-1 protease activity from Acacia pennata. Natural Products Communications13: 411-414.